Table 2.

Mutational effects on conformational stability (as folding free energy between the mutant and WT proteins, ΔΔG, in kcal·mol⁻¹, in the NQO1_holo and NQO1_dic states, determined by Rosseta). Negative values indicate a destabilizing effect whereas positive values indicate a stabilizing effect.

	G3S	G3D	L7P	L7R	V9I	T16M	Y20N	A29T
NQO1holo	−5.0	−11.6	−29.4	−14.1	1.9	−0.8	−8.1	−10.9
NQO1dic	−1.5	−10.1	−25.5	−35.9	0.6	0.8	−5.4	0.4