Figure 2.

Susceptibility of MAPK mutants to macrophage-mediated killing. Yeast cells were added to a confluent monolayer of murine macrophages from cell line RAW 264.7 at MOI 1:20 (yeast:m0) and co-incubated for 4 h at 37 °C and 5% CO₂. Killing percentages were assessed after CFU counting of fungal cells obtained upon interaction and related to fungal cells assayed without macrophages (positive control–0% killing). All strains were normalized to the wild type (CAF2) with a given value of 30% killing. Error bars represent the standard deviation of duplicates. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (one-way ANOVA, multiple comparison tests related to CAF2).