Figure 3.
Sensitivity of MAPK mutants to antifungal treatment. Cells from cultures grown overnight at 37 °C were inoculated in a 96-well plate at final O.D. = 0.025 (105 cells). Amphotericin B or fluconazole was added to the plates in increasing 2-fold concentrations ranging from 0.030 µg/mL to 15 µg/mL (shown only 0.94 µg/mL to 7.5 µg/mL-amphotericin B) or 0.32 µg/mL to 160 µg/mL (fluconazole). Inoculated plates were incubated at 37 °C for 24 h prior to O.D. assessment. Each strain has been normalized to its positive control (without antifungal) and the growth inhibition curve is shown. Error bars represent the standard error of the mean from two independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (two-way ANOVA multiple comparison tests related to CAF2).