Figure 2.

Anti-D4 IgG titres elicited by Swiss albino mice immunised via intraperitoneal (i.p.) route with PBS, D4 only, AH np/D4, alhydrogel/D4, NISV-D4, NISV-D4/alhydrogel, and NISVD4/AH np. Subsequent booster doses were administered on days 14 and 28. Sera were collected from individual mice and analysed in triplicate for D4-specific IgG antibodies. The results were expressed as mean values with standard deviation (SD) from each individual mice group. The p-value was calculated between AH np adjuvanted groups and alhydrogel using two-way Anova followed by Tukey’s multiple comparison tests. ns: non-significant, **** p < 0.0001.