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. 2020 Oct 1;8(4):571. doi: 10.3390/vaccines8040571

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Anti-D4 (a) IgG1 and (b) IgG2a titres elicited by Swiss albino mice immunised via IP route with PBS, D4 only, AH np/D4, alhydrogel/D4, NISV-D4, NISV-D4/alhydrogel, and NISV-D4/AH np. Subsequent booster doses were administered on days 14 and 28. Sera were collected from individual mice and analysed in triplicate for D4-specific IgG antibodies. The results were expressed as mean value with standard deviation (SD) from each individual mice group. p-value was calculated between AH np adjuvanted groups and alhydrogel using two-way Anova followed by Tukey’s multiple comparison tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns represents non-significant.