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Stimulation of HEK293-Blue cells expressing hTLR4 (A,C) or mTLR4 (B,D) with purified LPS (A,B) or whole-cell preparations of B213 and derivatives (C,D). LPS preparations and bacterial suspensions were serially diluted. After incubation for 2 h with HEK293-Blue cells expressing mTLR4 or for 4 h with HEK293-Blue cells expressing hTLR4, alkaline phosphatase activity was determined. Graphs show the mean and standard deviation from a representative experiment of three repeats in duplicate.
