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. 2020 May 18;11(1):105–115. doi: 10.1515/tnsci-2020-0105

Figure 4.

Figure 4

Restitution of Bax blocks the protective effect of miR-142-3p in hydrogen peroxide-exposed BV-2 cells. (a) The BV-2 cells were exposed to hydrogen peroxide of varied concentrations (50–400 μM) for 10 h followed by qRT-PCR evaluation of miR-142-3p expression. *P < 0.05, **P < 0.01 compared to the control group of cells. (b) qRT-PCR analysis for expression of miR-142-3p in BV-2 cells after transfection with miR-142-3p mimics or NC. **P < 0.01 compared to NC mimics. (c) BV-2 cells received transfection of pc-DNA-Bax or pc-DNA-vector followed by western blot analysis for expression of Bax. (d) Results of western blot analysis for expression of Bax in hydrogen peroxide-treated cells, which received transfection of miR-142-3p mimics or miR-142-3p and pc-DNA-Bax. After treatment with hydrogen peroxide, the cells were transfected with miR-142-3p-mimics or miR-142-3p-mimics and pc-DNA-Bax. (e) Quantitative results of % apoptotic cells. (f) Quantitative results of capase-3 activity. **P < 0.01, @P < 0.01. The results are presented as mean ± SD.