Table 3.

Results obtained with 35, 40, 45 and 50 amplification cycles using different concentrations of DNA of ten different species, with the respective panels. - means absence of amplification and +++ high, ++ medium and + low intensity.

Candida Panel						Filamentous Fungi Panel
Species	DNA	Amplification Cycles				Species	DNA	Amplification Cycles
		35	40	45	50			35	40	45	50
C. albicans	1 ng	+++	+++	+++	+++	A. fumigatus	1 ng	+++	+++	+++	+++
	100 pg	++	++	++	++		100 pg	++	++	+++	+++
	10 pg	-	+	++	++		10 pg	-	+	++	++
	1 pg	-	-	+	+		1 pg	-	+	+	+
C. glabrata	1 ng	+++	+++	+++	+++	A. flavus	1 ng	+++	+++	+++	+++
	100 pg	+++	+++	+++	+++		100 pg	+++	+++	+++	+++
	10 pg	-	++	+++	+++		10 pg	-	+	+	+
	1 pg	-	+	++	++		1 pg	-	-	-	-
C. parapsilosis	1 ng	++	++	++	++	A. niger	1 ng	+++	+++	+++	+++
	100 pg	++	++	++	++		100 pg	+++	+++	+++	+++
	10 pg	-	+	+	+		10 pg	-	+	++	++
	1 pg	-	-	-	-		1 pg	-	-	+	+
C. tropicalis	1 ng	++	++	++	++	A. terreus	1 ng	+++	+++	+++	+++
	100 pg	++	++	++	++		100 pg	++	+++	+++	+++
	10 pg	-	+	+	+		10 pg	-	-	+	+
	1 pg	-	-	-	-		1 pg	-	-	-	-
C. krusei	1 ng	+++	+++	+++	+++	R. arrhizus	1 ng	+++	+++	+++	+++
	100 pg	+++	+++	+++	+++		100 pg	+++	+++	+++	+++
	10 pg	-	+	++	++		10 pg	-	+	++	++
	1 pg	-	+	++	++		1 pg	-	-	-	-