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The 2-TT to reduce the false positive rate in expanded newborn screening for isovaleric acidemia (IVA). The contributions from different isobaric molecules can be separated by chromatography coupled with MS-MS as described in Poggiali et al. [26]. Interference causing false abnormal C5-acylcarnitine levels can be observed for pivalic acid supplementation or for 2-methylbutyrylglycinuria, a benign metabolic condition.
