Table 2.
Antibodies used for immunocytochemistry.
| Antibody | Company, Cat number | Dilution |
|---|---|---|
| Primary | ||
| Rabbit IgG anti-GABA | Sigma, ABN131 | 1:500 |
| Mouse IgM anti-PAX6 | Santa Cruz Biotechnology, sc-81,649 | 1:50 |
| Goat IgG anti-SOX1 | R&D Systems, AF3369 | 1:200 |
| Goat IgG anti-OTX2 | R&D Systems, AF1979 | 1.400 |
| Mouse IgG2a anti-TuJ1 | Covance, 801201 | 1:1000 |
| Rabbit IgG anti-NF200 | Sigma, N4142 | 1:1000 |
| Rabbit IgG anti-DARPP32 | Abcam, ab40801 | 1:50 |
| Chicken IgG anti-MAP2 | Abcam, ab5392 | 1:1000 |
| Rabbit IgG anti-SOX2 | Cell Signaling Technology, 3579 | 1:500 |
| Mouse IgM anti-TRA-1-60 | Abcam, ab16288 | 1:200 |
| Mouse IgG anti-NANOG | Abcam, ab62734 | 1:200 |
| Mouse IgG anti-OCT4 | BD Transduction Lab, 611,202 | 1:50 |
| Mouse IgG anti-αSMA | DAKO, M0851 | 1:100 |
| Rabbit IgG anti-HNF3B | Millipore, 07-633 | 1:200 |
| Rabbit IgG anti-HTT | Sigma, H7540 | 1:100 |
| Mouse IgG anti-PolyQ | Millipore, MAB1574 | 1:400 |
| Rabbit IgG anti-Cleaved caspase-3 | Cell Signaling Technology, 9661 | 1:400 |
| Mouse IgG1 anti-ZO-1 | Invitrogen, 33-9100 | 1:50 |
| Rabbit IgG anti-Synapsin I | Millipore, 574,777 | 1:200 |
| Secondary | ||
| Alexa Fluor 488 goat anti rabbit IgG (H+L) | Thermo Fisher Scientific, A11,078 | 1:400 |
| Alexa Fluor 568 goat anti rabbit IgG (H+L) | Thermo Fisher Scientific, A-11,011 | 1:400 |
| Alexa Fluor 488 goat anti mouse IgG (H+L) | Thermo Fisher Scientific, A28,175 | 1:400 |
| Alexa Fluor 568 goat anti mouse IgG (H+L) | Thermo Fisher Scientific, A11,031 | 1:400 |
| Alexa Fluor 568 goat anti mouse IgG2a | Thermo Fisher Scientific, A-21,134 | 1:400 |
| Alexa Fluor 488 goat anti mouse IgG1 | Thermo Fisher Scientific, A-21,121 | 1:400 |
| Alexa Fluor 568 goat anti mouse IgM | Thermo Fisher Scientific, A21,043 | 1:400 |
| Alexa Fluor 488 rabbit anti goat IgG (H+L) | Thermo Fisher Scientific, A27,012 | 1:400 |
| Alexa Fluor 488 goat anti chicken IgY H&L | Abcam, ab150,169 | 1:400 |