Table 3.
Top 27 off-target sites in the human genome for the spacer of the HTT-targeted CRISPR/Cas9 system.
| Sequence | PAM | Score | Chromo-Some | Gene | DNA Strand | Substitutions |
|---|---|---|---|---|---|---|
| AGGCCTTCATCAGCTTTTCC | AGG | 100 | 4 | HTT | - | 0 |
| AAGCCTTCATCATCTTTTCC | TGG | 3.60441176 | 2 | - | 2 | |
| CAGCTTTCATCAGCTTTTCC | AGG | 2.50329381 | 8 | + | 4 | |
| ATGATTTCATCAGCTTTTCC | GAG | 2.4265645 | 6 | - | 3 | |
| AGCCCTATATCAGCTTTTCC | CAG | 1.74086014 | 22 | + | 4 | |
| ATGCATTCTTCAGCTTTTCC | CAG | 1.68981077 | 12 | + | 3 | |
| GGGCCCTCAGCAGCTTTTCC | TGG | 1.65679108 | 5 | + | 3 | |
| AGCCTTTCATGAGCTTTTCC | CAG | 1.56823077 | 2 | - | 3 | |
| CAGCCTTCATCAGCTTCTCC | AAG | 1.55307856 | 22 | - | 3 | |
| CAGCTTTAATCAGCTTTTCC | TAG | 1.50316456 | 4 | - | 4 | |
| TGGTCTTAAGCAGCTTTTCC | TAG | 1.47132848 | 5 | + | 4 | |
| TTTCCTTCAACAGCTTTTCC | TGG | 1.41271949 | 11 | - | 4 | |
| AGTCCTCCATCAGCTTTTCT | AAG | 1.19365756 | 8 | + | 3 | |
| GAGCCTGGATCAGCTTTTCC | TGG | 1.04428648 | 14 | - | 4 | |
| TGGGCTGAATCAGCTTTTCC | TGG | 1.02666139 | 6 | + | 4 | |
| TGGCTTCCAGCAGCTTTTCC | AAG | 0.98720955 | 7 | - | 4 | |
| ATGGCTGCAGCAGCTTTTCC | AAG | 0.97011502 | X | - | 4 | |
| AAGAATCCATCAGCTTTTCC | AAG | 0.96173419 | 2 | + | 4 | |
| AATTCTACATCAGCTTTTCC | TAG | 0.94826991 | 9 | + | 4 | |
| AAATCTCCATCAGCTTTTCC | GAG | 0.94826991 | 6 | - | 4 | |
| TGCCCTTGGTCAGCTTTTCC | TGG | 0.94546867 | 20 | - | 4 | |
| AGGGCCTCATGAGCTTTTCC | AAG | 0.92863537 | 9 | + | 3 | |
| AGGGCTGTAGCAGCTTTTCC | CAG | 0.90727356 | 16 | + | 4 | |
| AGGGCTGTAGCAGCTTTTCC | CAG | 0.90727356 | 16 | + | 4 | |
| TAGCATTCATCAGCTTTTCA | AGG | 0.90034091 | 5 | + | 4 | |
| TAGCTTTCATCAGCTTTTCA | AAG | 0.90034091 | X | - | 4 | |
| TAGCTTTCATCAGCTTTTCA | AAG | 0.90034091 | 11 | + | 4 | |
| TTGCTTTCATCAGCTTTTCA | AAG | 0.90034091 | 22 | HMGXB4 | + | 4 |
PAM: protospacer-adjacent motif. The target protospacer is highlighted in green, and the studied off-target sites in orange.