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. 2020 Sep 28;6(4):193. doi: 10.3390/jof6040193

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HSP90 detection in fungal and mammalian protein extracts with mAb 4D11. (A) Western Blot with 4D11 monoclonal antibody (mAb) and cell surface protein extracts from P. lutzii (1), P. brasiliensis (2), C. albicans (3) and C. neoformans (4), Human embryonic kidney (HEK)293 human whole-cell protein extract (5) and recombinant P. lutzii HSP90 (6). (B) Similar experiment, but with cytosolic protein extracts from P. lutzii (1), P. brasiliensis (2), C. albicans (3) and C. neoformans (4) and recombinant P. lutzii HSP90 (5). MW: Molecular Weight standard.