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. 2020 Oct 3;7(4):150. doi: 10.3390/vetsci7040150

Figure 1.

Figure 1

CD4+CD25+ cells suppress the proliferation of naive T cells: (A) negative control: CD4+CD25− cells with no CFSE staining; (B) positive control: CD4+CD25− cells with CFSE staining; (C) ConA-stimulated CD4+CD25− cells; (D) coculture of ConA-stimulated CD4+CD25− cells with CD4+CD25+ cells at a ratio of 1:1. CFSE dilution of CFSE-labeled CD4+CD25− cells was measured at 66 h of coculture after gating on CFSE-stained cells. Three gates mean: no CFSE, the percentage of CFSE negative cells; CFSE, the percentage of no-proliferative CFSE positive cells; CFSE subset-1, the percentage of proliferative CFSE positive cell. n = 3, results are representative of two independent experiments.