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. 2020 Oct 3;7(4):150. doi: 10.3390/vetsci7040150

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The percentage of CD4⁺CD25⁺ cells by flow cytometry: (A) The lymphocytes were grouped and CD4⁺CD25⁺ cells were gated as shown in Figure 3A (up left: lymphocytes group; up right: isotype control; down left: CD25 staining; down middle CD25 staining; down right: CD4 and CD25 staining). (B) The percentage of CD4⁺CD25⁺ cells. The error bars indicate the SEM. * p ≤ 0.05; ** p ≤ 0.01.