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The growth property of reassortant viruses in serum-free medium. The multiplicity of infection (MOI) was 0.0001. TPCK-trypsin was supplied, and the samples were collected every day for Tissue Culture Infective Dose (TCID) assay and hemagglutination assay (HA). (a) The peak titer of virus growth in T75 flasks. (b) The peak titer of virus growth in spinner flasks.
