Figure 5.

Dectin-1 inhibition assay with laminarin. Expanded NK cells were treated with laminarin for 1 h before exposure to the Aspergillus morphotypes (swollen conidia—SC and Hyphae—H) for 6 h. (A) XTT assay showing killing of swollen conidia and hyphae by cells treated with laminarin compared to the untreated cells (* p < 0.05). (B) Perforin levels in cells treated with laminarin compared to untreated cells (* p < 0.05). (C) Granzyme B levels and (D) IFN-γ production following blockade of dectin-1 receptors with laminarin. Data shown is representative of the mean of duplicate readings (± SEM) pooled from 3 experiments.