Figure 5.
Detection of serum IgG and mucosal IgA antibodies induced upon ShigETEC vaccination. (a) Mice were vaccinated 3 times i.n. with 108 CFU ShigETEC. Specific IgG antibody levels were evaluated against indicated antigens in serum obtained 4 weeks after the last vaccination using the indicated serum dilutions in ELISA. Symbols represent averages of duplicate measurements of sera from individual mice (43 mice per group) from three independent vaccination experiments. (b) Mice were vaccinated 3 times i.n. with 108 CFU ShigETEC and challenged with lethal doses of either S. sonnei or S. flexneri 6 four weeks after the last vaccination. Bronchoalveolar lavages (BAL) were taken two weeks after the challenge. Specific IgA antibody levels were evaluated against the indicated antigens using the indicated serum dilutions in ELISA. Symbols represent averages of duplicate measurement of BAL samples from individual mice from three independent vaccination experiments with 18 and 41 mice per group for mock and ShigETEC, respectively.