Table 1.
Genes examined, primers used for RT‐PCR, and PCR products
| Gene | GenBank accession no. | Primers | PCR products |
|---|---|---|---|
| HIF‐1α | NM_181054.2 | Forward: ATCCATGTGACCATGAGGAAATG | 126 bp (nt 814–939) |
| Reverse: CTCGGCTAGTTAGGGTACACTT | |||
| HIF‐2α | NM_001430.4 | Forward: GTGCCATGACAAACATCTTCCAG | 107 bp (nt 2204–2310) |
| Reverse: CTCGGGCTCTGTCTTCTTGCT | |||
| VEGF | AF022375 | Forward: GTCCCAGGCTGCACCCATG | 158 bp (nt 522–679) |
| Reverse: AGGAAGCTCATCTCTCCTA | |||
| Flt‐1 | NM_001160031 | Forward: GTGCGCGCTGCTCAGCTG | 160 bp (nt 318–477) |
| Reverse: AGGCAAAGACCATTTATG | |||
| GLUT1 | K03195 | Forward: GAGTTCTACAACCAGACATGG | 167 bp (nt 302–468) |
| Reverse: GCATTGAATTCCGCCGGCCA | |||
| LDHA | X02152 | Forward: GCTGGTCATTATCACGGCTG | 128 bp (nt 361–488) |
| Reverse: AGCAACTTGCAGTTCGGGCTG | |||
| Cyclin D1 | NM_053056 | Forward: GCGCGCCCTCGGTGTCCTA | 159 bp (nt 322–480) |
| Reverse: GCGACAGGAAGCGGTCCAGG | |||
| TGF‐α | NM_003236 | Forward: GCTGCGTGCCAGGCCTTGGAG | 163 bp (nt 257–419) |
| Reverse: ACATGCTGGCTTGTCCTCCTG |