Figure 6.

Inhibition of p38 MAPKs did not alter the caspase 8 or caspase 3 activity in CRC cells. HCT-116 and HT-29 CRC cell lines were treated with different doses of losmapimod or doramapimod or DMSO (control) for 48 h. Whole cell extracts were prepared and subjected to SDS-PAGE. Western blotting was performed using specific antibodies to caspase 8 and caspase 3. Equal loading was confirmed with β-actin. As shown above, neither caspase 3 or caspase 8 levels were altered by losmapimod or doramapimod.