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. 2020 Dec 2;8(4):720. doi: 10.3390/vaccines8040720

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lack of inhibition of biliary glycoprotein (BGP) protein levels by losmapimod or doramapimod by in colon cancer cells. (a,b) HCT-116 and (c,d) HT-29 CRC cells were treated with different doses of losmapimod or doramapimod or DMSO (control) for 48 h. Whole cell extracts were prepared and SDS-PAGE was performed followed by Western blotting with specific BGP antibodies. Equal loading was confirmed with β-actin. Panels (b,d) depict the resultant densitometric values showing fold change of the ratio of BGP/β-actin in HCT-116 and HT-29 cells.