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. Author manuscript; available in PMC: 2021 Jun 23.
Published in final edited form as: J Vis Exp. 2020 Jun 23;(160):10.3791/60682. doi: 10.3791/60682

Figure 1: Iron treatment increases ROS in colorectal cancer cells.

Figure 1:

Representative fluorescent images taken by a fluorescence microscope and intensity quantification by a fluorescence microplate reader for 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) staining in HCT116 cells after untreated control (Ctrl), (A) 100 μM ferrous sulfate (FS) or (B) 10 μM doxorubicin (DOX) treatment for 24 h. Scale bar = 400 μm. * = p < 0.05; *** = p < 0.001.