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. 2020 Dec 1;8(4):715. doi: 10.3390/vaccines8040715

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cultured ELISPOT assay for antigen specific T_CM like cells. At 20 weeks after the last vaccination, lymphocytes of mice were cultured with or without a cocktail of antigens ESAT-6, Ag85B, Rv2626c and HspX for 9 days. Then, cells were re-stimulated with the same antigens for 20 h. (A) Representative images of IFN-γ ELISPOT wells from long-term cultured ELISPOT assays at 5× magnification. (B) Results of long-term cultured ELISPOT responses assay. Data were presented as means ± SD from groups of 4 mice. * p < 0.05.

Cultured ELISPOT assay for antigen specific T_CM like cells. At 20 weeks after the last vaccination, lymphocytes of mice were cultured with or without a cocktail of antigens ESAT-6, Ag85B, Rv2626c and HspX for 9 days. Then, cells were re-stimulated with the same antigens for 20 h. (A) Representative images of IFN-γ ELISPOT wells from long-term cultured ELISPOT assays at 5× magnification. (B) Results of long-term cultured ELISPOT responses assay. Data were presented as means ± SD from groups of 4 mice. * p < 0.05.