Figure 4.

Proliferative capability of lymphocytes. For proliferation assay, 20 weeks after the last vaccination, lymphocytes (5 × 10⁶ cells/well) were stimulated with a cocktail of antigens ESAT-6, Ag85B, Rv2626c and HspX (2 μg/mL) for 7 days in 24-well plates. Three days after antigen stimulation, EdU was added at a final concentration of 30 μM and cells were cultured for another 4 days. At the 7th day, proliferative cells were detected by flow cytometry. (A) The representative results of every group. (B) Frequency percentages of EdU+ in CD4⁺ T cells; (C) Frequency percentages of EdU+ in CD8⁺ T cells. Results are presented as means ± SD from groups of 5 mice. * p < 0.05.