Fig. 4.

Lack of IFNAR signaling on Tregs leads to defective recruitment of MDSCs to dLN during priming

EAE was induced in Foxp3^YFP-Cre (black spheres) or IFNAR^fl/fl Foxp3^YFP-Cre (red cubes) mice and (A) Percentages of CD90⁻/CD19⁻CD11b⁺ cells in draining lymph nodes and absolute number of G-MDSCs (CD90⁻/CD19⁻CD11b⁺Ly6G⁺Ly6C⁻) and M-MDSCs (CD90⁻/CD19⁻CD11b⁺Ly6G⁻Ly6C^hi) cells in (B) draining lymph nodes, (C) spleen, (D) CNS (brain and spinal cord) tissue and (E) bone marrow were measured at the indicated time points. N=3, mean± SE, representative of two independent experiments.

(F) Foxp3^YFP-Cre or IFNAR^fl/fl Foxp3^YFP-Cre mice were injected Edu (200mg/kg) i.p at d 0 and d 2, immunized with MOG-CFA (filled black circle=WT, filled red square=cKO) or left unimmunized (open black circle=WT, open red square=cKO), and euthanized on d 3. The incorporation of Edu in G-MDSCs and M-MDSCs from bone marrow was determined (N=5, mean± SE, representative of two independent experiments).

(G) IFNAR^fl/fl or IFNAR^fl/fl LysM^Cre mice were immunized with MOG-CFA and the percentages of G-MDSCs and M-MDSCs in dLN were quantitated on d 7 (N≥5, mean± SE, cumulative of two independent experiments.