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Detection of CCL35.2HA and ORF25HA protein in vivo. The gibel carp were intramuscularly injected with pcDNA3.1-neo, pcCCL35.2 and pcORF25, respectively. The muscle sections were carried out IFAs with mouse anti-HA antibody and goat anti-mouse IgG-FITC (green) second antibody. Cell nuclei were stained by DAPI (blue). The red arrow indicates the specific green fluorescence produced by indirect immunoassay.
