Figure 4.

IgM levels in tissue and serum. (A) Vaccination/challenge and sampling schedule. mRNA expression patterns of IgM in head kidney (B) and spleen (C) were determined by qRT-PCR. β-actin gene was used as an internal control gene. (D) Detection of serum antibody levels by ELISA in gibel carp. Different superscript letters in each group (a–c) denote significant variations suggested by the Kruskal–Wallis statistics at 95% of significance, followed by the Dunn test with Bonferroni adjustment as the post hoc test (p < 0.05).