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mRNA expression patterns of representative important immune regulation and effector genes in head kidney and spleen tissues. IL-1β (A,B), IL-2 (C,D) transcripts were examined by qRT-PCR. β-actin gene was used as an internal control gene. Different superscript letters in each group (a–d) denote significant variations suggested by the Kruskal–Wallis statistics at 95% of significance, followed by the Dunn test with Bonferroni adjustment as the post hoc test (p < 0.05).
