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. 2020 Oct 1;8(4):570. doi: 10.3390/vaccines8040570

Figure 1.

Figure 1

DNA electroporation of influenza antigens: in vivo antigen expression and immunogenicity. (A) Long-term antigen expression after DNA electroporation. BALB/c mice (n = 2–3) were intramuscularly electroporated with 20 µg of the luciferase-encoding plasmids pDP-LUC-empty, pDP-LUC-HA or pDP-LUC-NP. Luminescence signals were quantified in the hind legs of immunized mice at indicated time-points after electroporation. The dotted line represents background luminescence. (B) Two BALB/c mice were intramuscularly electroporated in both hind legs with 20 µg luciferase-encoding plasmid pDP-LUC-empty, three BALB/c mice received an intramuscular electroporation of 20 µg pDP-LUC-NP in the left hind leg and 20 µg pDP-LUC-HA in the right hind leg. Luminescence signals were quantified in the red-circled areas, here shown 30 days after electroporation. Background luminescence is shown in ROI = 5.