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. 2020 Oct 1;8(4):570. doi: 10.3390/vaccines8040570

Figure 3.

Figure 3

Soluble checkpoint co-expression modulates HA-specific antibody responses. (A) Six-week old BALB/c mice were electroporated intramuscularly with expression plasmids encoding for HA and NP together either with an empty vector (mock) or plasmids encoding for sPD-1 or sPD-L1. Four weeks after priming, a booster immunization was administered Blood was drawn at weeks 3, 6 and 18 and antibody responses were analyzed by ELISA. NP-specific IgG (B), IgG1 (C) and IgG2a (D) antibody responses and HA-specific IgG (E), IgG1 (F) and IgG2a (G) antibody responses in the sera of BALB/c mice after i.m. electroporation over a time-period of 18 weeks. Shown are mean values with SEM (n = 9–18) and significant differences between immunized groups (two-way ANOVA analyses followed by Tukey’s multiple comparison test, (F) * p < 0.05 for sPD-L1 group compared to sPD-1 group, **** p < 0.0001 for sPD-1 group compared to mock).