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. 2020 Oct 1;8(4):570. doi: 10.3390/vaccines8040570

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PD-1 co-expression results in a more balanced HA-specific antibody response. Antibody subtype patterns of HA-immunized mice three weeks after priming and two (week 6) and 14 weeks (week 18) after the booster immunization. The ring size represents the overall antibody response. Shown are mean percentages of 18 animals from three independent experiments. Each subtype was analyzed by ELISA with identical amounts of HRP-conjugated anti-mouse IgG1 (blue), IgG2a (red), IgG2b (gray), and IgG3 (black) antibodies.