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. 2020 Oct 26;6(4):248. doi: 10.3390/jof6040248

Figure 2.

Figure 2

Pex6 is required for hyphal growth, conidia germination and the formation of appressorium -like structures in A. alternata. (A) Deletion of pex6 results in growth retardation. Fungal strains: wild type (WT), two pex6 mutants (Δpex6-M4 and M9) and the complementation strain (CP4) were grown on potato dextrose agar (PDA) or minimal medium (MM) for 3 days; (B) Deletion of pex6 plays no roles in conidia formation. Bar = 50 µm; (C) Conidia of both M4 and M9 strains germinate at rates much slower than those of wild type and CP4; (D) The formation of appressorium-like structures (nonmelanized enlargement of hyphal tips, indicated by arrows) by wild type on microtiter plates; (E) When culturing in liquid MM, Δpex6 produces fewer appressorium-like structures on microtiter plates. Significance of difference between means was analyzed using Tukey’s HSD post-hoc test (p ≤ 0.05). Means (n = 100) followed by the same letters are not significantly different.