Figure 3.

Pex6 is required for cell viability in A. alternata. (A) Assays for the viability of conidia prepared from wild type (WT), two pex6 mutants (Δpex6-M4 and M9) and the complementation strain (CP4) by Evan’s blue staining. Live cells are capable of expelling dye and remain clear, whereas dead cells fail to expel dye and are stained blue; (B) Percentage of unstained conidia collected from fungal strains were incubated in potato dextrose broth (PDB) or minimal medium (MM) for 7 h. Significance of difference between means was analyzed using Tukey’s HSD post-hoc test (p ≤ 0.05). Means (n = 100) followed by the same letters are not significantly different.