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. 2020 Oct 26;6(4):248. doi: 10.3390/jof6040248

Figure 4.

Figure 4

Pex6 is involved in lipid metabolism and maintenance of cell-wall integrity in A. alternata. (A) Assays for sensitivity of wild type (WT), two pex6 mutants (Δpex6-M4 and M9) and the complementation strain (CP4) to oleic acid (OA, 0.5%), Tween 20 (Tw20, 0.5%), calcofluor white (CFW, 200 µM) and Congo red (CR, 50 µM) on potato dextrose agar (PDA) or minimal medium (MM); (B) Quantitative analysis of chemical sensitivity on PDA. Significance of differences was analyzed using Tukey’s HSD post-hoc test. Means indicated by asterisks are significantly different from wild type, p < 0.01 (**), p <0.05 (*); (C) Percentage of growth reduction of Δpex6 in relation to wild type grown on PDA amended with different concentrations of oleic acid for 3 days; (D) Nile red staining of fungal hyphae and conidia for the presence of lipid bodies showing blight red fluorescence.