Figure 6.

Pex6 is required for A. alternata pathogenicity and lesion formation. (A) Fungal pathogenicity was assayed on detached calamondin leaves; (B) Fungal pathogenicity was assayed on citrus leaves that were wounded before inoculation. Each spot was inoculated with 5 µl of conidial suspensions (10⁵ conidia per milliliter) prepared from the wild-type (WT) two pex6 mutants (Δpex6-M4 and M9) or the complementation strain (CP4). The leaves were maintained in a moisture chamber for lesion development. M4 and M9 induced much smaller lesions (indicated by red arrows). Each strain was tested on at least five different leaves and experiments were repeated at least three times.