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Pex6 deficiency leads to penetration impairment. Calamondin leaves were inoculated with conidia prepared from wild type (WT) and the Δpex6-M4 mutant, thin sectioning by hands 1 to 3 days post inoculation (dpi), and observed by microscopy. Wild-type hyphae (indicated by white arrows) after lactophenol cotton blue staining were observed in the epidermal layer and mesophyll cells. Hyphae of the Δpex6-M4 mutant were observed only in the epidermal layer of citrus leaves. Bar = 20 µm.
