Figure 10.

Partial restoration of Δpex6 virulence with purified toxin. ACT (~12–15 µg toxin/g mycelium) was purified from culture filtrates of wild type grown in potato dextrose broth for 7 days using Amberlite XAD-2 resins and ethyl acetate, dissolved in methanol, diluted (10×, 100×, or 1000× in methanol), and applied onto calamondin leaves that were wounded before treatments. After 30 min, the leaves were inoculated with conidial suspensions prepared from wild type (WT) and the Δpex6-M4 mutant. The leaves were maintained in a moisture chamber for 3 days for lesion development.