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Representation of the preparation and sensing process: (1) Loading and functionalization of the nanoporous anodic alumina (NAA) support; (2) Capping with the oligonucleotides O1, O2, or O3, to obtain solids S1, S2, and S3, respectively; (3) Release of the cargo in the presence of P. jirovecii genomic DNA by forming (A) duplex structures when using solids S1 or S3 or (B) triplex hybridization structures when using S2 and (4) Calcination prior to the reutilization of the scaffold.
