Table 1.
Overview of the 29 patients with dermatomycoses due to T. mentagrophytes of (ITS) genotype VIII (India) diagnosed all over Germany from 2016–2020. Abbreviations: SQLE, squalene epoxidase; ITS, internal transcribed spacer; TEF1-α, translation elongation factor 1-α; MIC, minimum inhibitory concentration; NCBI, National Center for Biotechnology Information, Bethesda, Maryland; DSM, Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures), Braunschweig, Germany; f, female; m, male.
| Strain Number | Collection No. DSMZ | Gender | Age in Years/ Months |
Pathology | Additional Information/ Remarks |
MIC Terbinafine [µg/mL] | Drug Resistance to Terbinafine | Amino Acid Substitution within the Squalene Epoxidase | Codon Change in SQLE | MIC itraconazole [µg/mL] | Drug Resistance to Itraconazole | MIC Voriconazole [µg/mL] | Drug Resistance to Voriconazole | GenBank Accession Number ITS Gene | GenBank Accession Number TEF1-α Gene (NCBI) | GenBank Accession Number SQLE Gene (NCBI) |
Sample Date | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 214677/16 | DSM 108903 | f | 35 | Tinea pedis | Indian | 0.2 | resistant | Phe397Leu | TTC → CTC | 0.06 | sensitive | 0.03 | sensitive | MT330252 | MT340499 | MT700499 | 2016 |
| 2 | 216377/17 | DSM 108902 | f | 29 | Tinea | Foreigners | 0.2 | resistant | Phe397Leu | TTC → CTC | 0.06 | sensitive | 0.03 | sensitive | MT330249 | MT340500 | MT700500 | 2017 |
| 3 | 211542/18 | DSM 108901 | m | 19 | Tinea corporis | Asylum office Herford | 8 | resistant | Phe397Leu | TTC → CTC | 0.087 | sensitive | 0.088 | sensitive | MT330250 | MT340501 | MT700501 | 2018 |
| 4 | 211564/18 | DSM 108900 | f | 30 | Tinea unguium et corporis | Indian | <0.2 | sensitive | Ala448Thr | GCT → ACT | 0.067 | sensitive | 0.088 | sensitive | MT330248 | MT340502 | MT700502 | 2018 |
| 5 | 218160/18 | DSM 108899 | m | 35 | Tinea corporis et cruris | Indian | <0.2 | sensitive | wild type | - | 0.125 | sensitive | 0.03 | sensitive | MT330253 | MT340503 | MT700503 | 2018 |
| 6 | 218360/18 | DSM 108898 | f | 25 | Tinea corporis | married couple on a pilgrimage in Saudi Arabia | <0.2 | sensitive | Ala448Thr | GCT → ACT | 0.5 | resistant | 0.5 | resistant | MT330255 | MT340504 | MT700504 | 2018 |
| 7 | 218691/18 | DSM 108897 | m | 58 | Tinea faciei | Visit India | 8 | resistant | Phe397Leu | TTC → CTC | 0.125 | sensitive | 0.03 | sensitive | MT330254 | MT340505 | MT700505 | 2018 |
| 8 | 901538/18 | DSM 108896 | m | 4 | Tinea corporis | German name | <0.2 | sensitive | Ala448Thr | GCT → ACT | 0.25 | sensitiv | 0.25 | resistant | MT330251 | MT340506 | MT700506 | 2018 |
| 9 | 200618/19 | DSM 109024 | f | 6 months | Tinea corporis | Family came from Bahrain to visit Germany | 0.2 | resistant | Phe397Leu | TTC → TTA | not applied | not applied | MT330285 | MT345568 | MT700507 | 2019 | ||
| 10 | 202953/19 | DSM 109747 | m | 27 | Tinea | Libyan, for three years in Germany, German girlfriend also affected | 8 | resistant | Phe397Leu | TTC→CTC | 0.5 | resistant | 0.25 | resistant | MT330284 | MT340507 | MT700508 | 2019 |
| 11 | 203513/19 | DSM 109748 | f | 25 | Tinea cruris et inguinalis | Pakistani | <0.2 | sensitive | wild type | - | 0.0312 | sensitive | 0.0312 | sensitive | MT330280 | MT340508 | MT700509 | 2019 |
| 12 | 600174/19 | DSM 109749 | f | 34 | Tinea corporis | Indian, was in India when the skin symptoms started | <0.2 | sensitive | Ala448Thr | GCT → ACT | 0.125 | sensitive | 0.0312 | sensitive | MT330279 | MT340517 | MT700510 | 2019 |
| 13 | 205666/19 | DSM 109751 | m | 32 | Tinea cruris | Iraqi couple (see no. 14) | <0.2 | sensitive | Ala448Thr | GCT → ACT | 0.125 | sensitive | 0.125 | sensitive | MT330281 | MT340516 | MT700511 | 2019 |
| 14 | 205667/19 | DSM 109750 | f | 31 | Tinea corporis | Iraqi couple (see no. 13) | <0.2 | sensitive | Ala448Thr | GCT → ACT | 0.5 | resistant | 0.5 | resistant | MT330283 | MT340515 | MT700512 | 2019 |
| 15 | 208737/19 | DSM 110678 | f | 29 | Tinea corporis | Indian name | 8 | resistant | Phe397Leu | TTC → TTA | not applied | - | not applied | - | MT328783 | MT340514 | MT700513 | 2019 |
| 16 | 209934/19 | DSM 110677 | m | 28 | Tinea corporis gluteal | Indian name | <0.2 | sensitive | Ala448Thr | GCT → ACT | not applied | - | not applied | - | MT330278 | MT340513 | MT700514 | 2019 |
| 17 | 600231/19 | DSM 110676 | m | 40 | Tinea corporis | Family, large plaques | 16 | resistant | Leu393Phe | TTA → TTC | not applied | - | not applied | - | MT330282 | MT340512 | MT700515 | 2019 |
| 18 | 214174/19 | DSM 110675 | m | 37 | Tinea faciei and corporis: cheeks, neck, forehead, and ear helix | German | 8 | resistant | Phe397Leu Ala448Thr |
TTC → CTC GCT → ACT | not applied | - | not applied | - | MT330289 | MT340511 | MT700516 | 2019 |
| 19 | 216532/19 | DSM 110674 | m | 27 | Tinea corporis | Indian | <0.2 | sensitive | Ala448Thr | GCT → ACT | not applied | - | not applied | - | MT330288 | MT340510 | MT700517 | 2019 |
| 20 | 217201/19 | DSM 110673 | m | 24 | Tinea corporis | Indian | <0.2 | sensitive | Val444 Ile Ala448Thr |
GTA → ATAGCT → ACT | not applied | - | not applied | - | MT330286 | MT340509 | MT700518 | 2019 |
| 21 | 218676/19 | - | m | 20 | Tinea corporis | Foreigner | <0.2 | sensitive | wild type | - | not applied | - | not applied | - | MT330256 | MT340518 | MT700519 | 2019 |
| 22 | 219238/19 | - | f | 27 | Tinea corporis | Pakistani | <0.2 | sensitive | wild type | - | not applied | - | not applied | - | MT330290 | MT340519 | MT700520 | 2019 |
| 23 | 600380/19 | - | - | Tinea | Indian | <0.2 | sensitive | wild type | - | not applied | - | not applied | - | MT330291 | MT340520 | MT700521 | 2019 | |
| 24 | 220575/19 | - | f | 24 | Tinea corporis gluteal | Student from Bangladesh | <0.2 | sensitive | wild type | - | not applied | - | not applied | - | MT330287 | MT340521 | MT700522 | 2019 |
| 25 | 600002/20 | - | m | Tinea cruris | German patient with stay in Thailand and India | 16 | resistant | Phe397Leu | TTC → CTC | not applied | - | not applied | - | MT333227 | MT340522 | MT700523 | 2020 | |
| 26 | 101549/20 | - | m | 30 | Tinea | Foreigner | 16 | resistant | Phe397Leu Ala448Thr |
TTC → CTCGCT → ACT | not applied | - | not applied | - | MT333225 | MT340523 | MT700524 | 2020 |
| 27 | 200874/20 | - | m | 27 | Tinea corporis et manum | Foreigner | <0.2 | sensitive | wild type | not applied | - | not applied | - | MT333228 | MT345569 | MT700525 | 2020 | |
| 28 | 900138/20 | - | m | 51 | Tinea corporis | Foreigner | 16 | resistant | Phe397Leu | TTC → CTC | not applied | - | not applied | - | MT333226 | MT340524 | MT700526 | 2020 |
| 29 | 204532/20 | - | f | 23 | Tinea corporis | Originating and migrating for studying from Bangladesh, her father also affected. She was pretreated in her home country by oral voriconazole, without success. | 0.2 | resistant | Phe397Leu | TTC → CTC | not applied | - | not applied | - | MT333242 | MT340525 | MT700527 | 2020 |