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. 2020 Nov 16;8(4):682. doi: 10.3390/vaccines8040682

Figure 1.

Figure 1

Figure 1

At every time-point post infection (pi), individual mouse sera, as well as, pooled equal volumes of sera of each mouse group were used, in parallel, with similar results. Mean values for the individual sera did not vary from pooled samples except for higher SD and are represented here (A–H). Antibody reactivity of individual naïve control mice to SmCB1 and SmCL3 at each time-point was negligible except for the IgM isotype, with a mean absorbance of 0.15 ± 0.05 and 0.03 ± 0.01 for SmCB1 and SmCL3, respectively. Each point represents the mean absorbance of quadruplicate wells (duplicate wells of 2 independent assays) of 1:200 diluted sera of 6 individual mice +/-SD. IgA antibodies binding to SmCB1 were only detected in 1: 25 diluted sera of SmCB1-immunized mice on day 17 (naïve, 0.081 ± 0.005; SmCB1, 0.161 ± 0.008), and days 24 and 40 (0.096 ± 0.001) pi. IgE antibodies binding to SmCB1 or SmCL3 were not detected at any time-point in any individual or pooled serum samples, diluted 1:25. ANOVA tests were used to analyze the statistical significance of differences between the three groups of mice, p < 0.05 (*) and < 0.005 (**).