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. 2020 Apr 28;10(5):855. doi: 10.3390/nano10050855

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Detection of reporter (lacZ) gene overexpression in hMSCs transduced with the rAAV/CD systems. The rAAV-lacZ vector (20 μL, 4 × 10⁵ transgene copies) was formulated with the various CDs (CD-1 to CD-4; 20 μL), and the resulting rAAV/CD systems (40 μL, i.e., 4 × 10⁵ transgene copies) were incubated with hMSCs (3000 cells in 96-well plates; MOI = 133) for up to 10 days. Expression of lacZ was examined using X-Gal staining (top panel: magnification ×4; scale bars: 500 μm; all representative data) with corresponding histomorphometric analyses (bottom left panel) and using quantitative estimation of the β-gal activities using the Beta-Glo^® Assay System (bottom right panel) after one (A) and 10 days (B). Control conditions included CDs lacking rAAV (-/CD), free rAAV (rAAV-lacZ), and absence of both CD and rAAV (-). Statistically significant relative to ^a -, ^b -/CD and ^c rAAV-lacZ.

Detection of reporter (lacZ) gene overexpression in hMSCs transduced with the rAAV/CD systems. The rAAV-lacZ vector (20 μL, 4 × 10⁵ transgene copies) was formulated with the various CDs (CD-1 to CD-4; 20 μL), and the resulting rAAV/CD systems (40 μL, i.e., 4 × 10⁵ transgene copies) were incubated with hMSCs (3000 cells in 96-well plates; MOI = 133) for up to 10 days. Expression of lacZ was examined using X-Gal staining (top panel: magnification ×4; scale bars: 500 μm; all representative data) with corresponding histomorphometric analyses (bottom left panel) and using quantitative estimation of the β-gal activities using the Beta-Glo^® Assay System (bottom right panel) after one (A) and 10 days (B). Control conditions included CDs lacking rAAV (-/CD), free rAAV (rAAV-lacZ), and absence of both CD and rAAV (-). Statistically significant relative to ^a -, ^b -/CD and ^c rAAV-lacZ.