Figure 11.

pH dependent release of DOX from CA (20 mM Ca²⁺), low Fe/Mg-CA (20 mM Ca²⁺, 5 µM Fe³⁺ and 20 mM Mg²⁺) and high Fe/Mg-CA (20 mM Ca²⁺, 25 µM Fe³⁺ and 60 mM Mg²⁺) NPs. DOX (5 µM) was used for each particle preparation in 200 µL freshly prepared DMEM media prior to incubation at 37 °C for 30 min. Then, 800 µL of DMEM media of different pHs (7.5, 7, 6.5, 6 and 5.5) was added to each sample.