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. 2020 Apr 27;10(5):834. doi: 10.3390/nano10050834

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell viability assessment for free DOX, DOX-loaded CA, DOX-loaded low Fe/Mg-CA and DOX-loaded high Fe/Mg-CA by thiazolyl blue tetrazolium bromide (MTT) assay in Michigan Cancer Foundation-7 (MCF-7) cells after 48 h of treatment. Results were considered significant at p-value 0.01–0.05 (*), very significant at p-value 0.001–0.01 (**), highly significant at p-value 0.0001–0.001 (***) and extremely significant at p-value ˂ 0.0001 (****). p values were calculated with respect to control (DOX in media) and treatment (DOX-bound NPs). The confidence interval was 95% for the respective samples.