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Biological function profiling of corona proteins (expressed as %) (A) CA (4 mM Ca2+). (B) Low Fe/Mg-CA (4 mM Ca2+, 1 µM Fe3+ and 4 mM Mg2+). (C) High Fe/Mg-CA (4 mM Ca2+, 5 µM Fe3+ and 12 mM Mg2+). After incubation and supplementation with 10% FBS, the particles were again incubated for 20 min at 37 °C and centrifuged twice at 13,000 rpm for 15 min. The pellets were then dissolved in 100 µL of 50 mM ethylene diamine tetraacetic acid (EDTA) in H2O.
