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. 2020 Dec 3;10:21192. doi: 10.1038/s41598-020-78292-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Haematoxylin and eosin staining of tissue integrity over 20 days observing spongiosis, necrosis, parakeratosis and epidermal/dermal separation. Representative images from Donor 1 haematoxylin and eosin stained tissue over time course. Days 1, 5, 7, 9, 13, 15 and 20 exhibited at × 20 magnification (scale bar = 100 µm). Days 1, 7, 13 and 20 exhibited at × 40 magnification (scale bar = 50 µm). Solid arrow points to spongiosis (Day 13). Empty arrow points to necrosis (Day 20). Solid triangle points to parakeratosis (Day 20).