Figure 1.
Genetic analysis of abnormal brown film formation in L. edodes cultivar, Chamaram. (a) Surface of sawdust cultivation media colonized with Chamaram and Sanjo701 (SJ701) cultivars after light exposure at the early vegetative mycelial growth stage. The abnormal brown mycelial film formation is indicated by the arrow. (b) Microstructure of SJ701 and Chamaram mycelial films. Scale bar = 20 μm. (c) Genetic analysis of abnormal brown film formation in the Chamaram cultivar using dihybrid lines. The karyotype 1, hypothetical genotype 2 related to mycelia browning pattern, phenotypes 3 of mycelia (B: normal brown film, AB: abnormal partial brown film) were represented. The putative dominant monokaryotic P37-5 line was crossed with the five indicated recessive monokaryotic strains. The brown film phenotypes of the heterozygotic dikaryon strains (P1–P5) are shown (right).