Figure 3.

Generation of monokaryotic strains overexpressing abl-D-GFP by protoplast transformation (a) Regeneration of L. edodes protoplasts. Scale bar = 10 μm. (b) Stable expression of GFP under the control of the native Legpd promoter. GFP fluorescence was observed at 15 h (Protolast) and 3 days (Regenerated hyphae) after transformation. Scale bar = 50 μm. (c–e) Expression analysis of the abl-D-GFP transgene. GFP fluorescence was observed in protoplasts ((c), left) and regenerated hyphae (d). Scale bar = 50 μm. PCR amplification of the transgene from the genomic DNA of transgenic mycelia and fruit bodies ((c,) right), and from cDNA derived from the mRNA of transgenic mycelia (e).