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. 2020 Dec 3;10:21109. doi: 10.1038/s41598-020-77987-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Appearance of A-LPS-bound PorA in co-culture of the porK and porR mutants. (A) OMV fractions of cultures of the wild type, ΔporA, porK, porU, ΔporR, and porU ΔporR strains and co-cultures of the porK and ΔporR strains, and the porK and porU ΔporR strains were subjected to SDS-PAGE, followed by immunoblot analyses using α-PorA, α-Hbp35, and mAb 1B5. CBB: Coomassie Brilliant Blue staining. The black arrow indicates the 23-kDa CTD-containing PorA. (B) Cell lysates (left) and OMV-free culture supernatants (right) of the wild type, ΔporA, ΔporR, wbpB, porK, porT, and porU strains were subjected to SDS-PAGE, followed by immunoblot analysis with α-PorA, respectively. The black and red arrows indicate the 23-kDa CTD-containing PorA and the 14-kDa CTD-lacking PorA, respectively.