Table 1.
In vitro specificity analyzes of the FOP and FOM probe species.
| DNA Test | Species | Collection Number | Source/Geography | Padlock Probe | |
|---|---|---|---|---|---|
| FOP | FOM | ||||
| Fungal DNA | F. pedrosoi | CBS271.37 T | Chromoblastomycosis, South America | (+) | (−) |
| F. monophora | CBS269.37 T | Chromoblastomycosis, South America | (−) | (+) | |
| F. erecta | CBS125763 T | Spine of Japecanga plant, Brazil, Bacabeira | (−) | (−) | |
| F. nubica | CBS125.198 T | Chromoblastomycosis, Cameroon | (−) | (−) | |
| F. pugnacious | CMRP1343 T | Chromoblastomycosis, South America | (−) | (−) | |
| C. albicans | CMRP816 | Human, Brazil | (−) | (−) | |
| P. citrinum | CMRP1538 | Metal, Tucuruí, Brazil | (−) | (−) | |
| A. nidulans | CMRP2338 | - | (−) | (−) | |
| Environmental test DNA samples | M. pudica | Plant in vitro | (−) | (−) | |
| M. pudica with F. pedrosoi | CBS271.37 T | Plant in vitro (20 ng/µL) with 0.5 ng/µL of F. pedrosoi | (+) | (−) | |
| M. pudica with F. monophora | CBS269.37 T | Plant in vitro (20 ng/µL) with 0.5 ng/µL of F. monophora | (−) | (+) | |
| M. pudica with F. erecta | CBS125763 T | Plant in vitro (20 ng/µL) with 0.5 ng/µL of F. erecta | (−) | (−) | |
| B. gasipaes with F. pedrosoi | CBS271.37 T | Plant inoculated with 105 spores of F. pedrosoi | (+) | (−) | |
| B. gasipaes with F. monophora | CBS269.37 T | Plant inoculated with 105 spores of F. monophora | (−) | (+) | |
| B. gasipaes with F. erecta | CBS125763 T | Plant inoculated with 105 spores of F. erecta | (−) | (−) | |
T, type strain; FOP, Fonsecaea pedrosoi padlock probe; FOM, Fonsecaea monophora padlock probe; (−), negative sample; (+), positive sample.