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. 2020 Oct 2;8(4):577. doi: 10.3390/vaccines8040577

Table 1.

Virus isolation from nasal swabs and leukocytes after the BVDV-1 challenge infection. The cattle were vaccinated with one (1x) or two (2x) shots of the candidate vaccine. BVDV-susceptible KOP-R cell cultures were inoculated with four replicates of fluids from nasal swabs (A) and purified leukocytes (B) from EDTA-blood samples (100 µL per replicate). The virus replication was verified by immunofluorescence staining 3 days p.i.. Results were scored according to the number of positive inoculations out of the 4 replicates (0 = no BVDV isolation, 4 = all inoculations BVDV positive) and visualized using light (1) to dark grey (4) colored fields. A first result was confirmed after one blind passage of the supernatant of the first cell culture inoculation.

(A) Virus Isolation from Nasal Swabs
Group Animal No. Days post challenge infection Cumulated
1 2 3 4 5 6 7 8 9 10 11 12 13 14 Scoring values
Unvaccinated 401 0 1 3 1 4 4 4 2 0 0 0 0 0 0 19
control 403 4 3 0 1 2 3 2 1 0 0 0 0 0 0 16
407 1 4 2 1 1 1 2 2 0 0 0 0 0 0 14
413 1 4 4 1 3 4 3 0 0 0 0 0 0 0 20
876 0 4 1 3 1 4 2 1 0 0 0 0 0 0 16
BVDV-1b_synCP7_ 406 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
ΔNpro_Erns Bungo 1x 418 3 4 3 0 1 0 1 0 0 0 0 0 0 0 12
893 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
894 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
898 0 0 0 0 3 0 0 0 0 0 0 0 0 0 3
BVDV-1b_synCP7_ 405 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
ΔNpro_Erns Bungo 2x 410 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
412 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
414 0 2 0 0 1 0 0 0 0 0 0 0 0 0 3
417 0 0 0 0 3 0 0 0 0 0 0 0 0 0 3
(B) Virus isolation from leukocytes
Group Animal No. Days Post Challenge Infection Cumulated
1 2 3 4 5 6 7 8 9 10 11 12 13 14 Scoring Values
Unvaccinated 401 0 0 1 1 4 4 3 2 1 1 0 0 0 0 17
control 403 0 2 0 1 2 4 2 1 0 0 0 0 0 0 12
407 0 2 2 0 4 4 1 0 1 1 1 0 0 0 16
413 0 4 1 4 4 4 3 1 0 0 0 0 0 0 21
876 0 1 0 1 3 4 0 1 0 0 0 0 0 1 11
BVDV-1b_synCP7_ 406 0 0 0 2 0 0 0 0 0 0 0 0 0 0 2
ΔNpro_Erns Bungo 1x 418 0 0 0 0 3 0 0 0 0 0 0 0 0 0 3
893 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
894 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
898 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
BVDV-1b_synCP7_ 405 0 1 0 0 1 0 0 0 0 0 0 0 0 0 2
ΔNpro_Erns Bungo 2x 410 0 0 0 0 3 1 0 0 0 0 0 0 0 0 4
412 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
414 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2
417 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1