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. 2020 Oct 22;7(4):133. doi: 10.3390/bioengineering7040133

Figure 4.

Figure 4

Characterization of myofibers differentiated from human iPSCs. (A) Expression of AchRs in a Day 15 SKM culture was demonstrated by staining with BTX-448. Myotubes were visualized with Myosin Heavy Chain (MHC) immunostaining. Scale bar: 100 µm. (B,C) Immunostaining of these myotubes for Ryanodine Receptor (RyR) (B) and Dihydropyridine Receptors (DHPR) (C) to examine the formation of excitation-contraction coupling in a Day 15 culture. Scale bars are 25 µm and 50 µm in (B) and (C), respectively.